This study examined the adhesive stability of screw-retained implant-supported fixed dental prostheses (FDPs) bonded to prefabricated gold cylinders after a load cycling. Five FDP groups (n = 10 per group) that differed either in bonding or loading modality (no loading, loading, moment loading, humidity, silicoating) were compared based on the forces needed to separate the FDP frameworks from the gold cylinders. The mean separating forces for the different groups ranged from 311 N to 501 N. No statistically significant differences could be detected between the five groups (Welch t test, P = .447). Despite the limited transferability into clinical practice, it appears that sufficient bonding stability can be achieved for screw-retained implant-supported FDPs bonded to prefabricated gold cylinders.