Objective: To analyse comparatively flow rate, pH, buffer capacity and biochemistry of the mixed saliva in miniature pigs (minipigs), Sprague-Dawley (SD) rats and humans. Materials and Methods: Twelve minipigs, 10 rats, and 16 human subjects were selected for tests of stimulated mixed saliva flow rate, pH, buffer capacity, and biochemistry. Results: Mixed saliva flow rate of minipigs was 1.401 ± 0.387 ml/min, similar to those of humans (1.183 ± 0.869 ml/min, p > 0.05). The pH and buffer capacity were higher in the minipigs' mixed saliva than in the humans'mixed saliva. There were some differences in the mixed saliva biochemistry indices among minipigs, rats, and humans. Conclusions:The minipig is considered a good animal candidate for saliva research. The characteristics of mixed saliva of minipigs and rats can be used in the selection of animal models for dental research. Schlagwörter: biochemistry, buffer capacity, miniature pig, pH, rat, saliva flow rate