This study evaluated the effects of enamel matrix derivative (EMD) and basic fibroblast growth factor (bFGF) with β-tricalcium phosphate (β-TCP) on periodontal healing in intrabony defects in dogs. One-wall intrabony defects created in dogs were treated with β-TCP alone (β-TCP), EMD with β-TCP (EMD/β-TCP), bFGF with β-TCP (bFGF/β-TCP), and a combination of each (EMD/bFGF/β-TCP). The amount of new bone formation was not significant for any group. The EMD/bFGF/β-TCP group induced significantly greater new cementum formation than the β-TCP and bFGF/β-TCP groups and, although not significantly, formed more new cementum than the EMD/β-TCP group. These findings indicate that EMD/bFGF/β-TCP treatment is effective for cementum regeneration.