The principle of guided tissue regeneration has demonstrated a level of success in regenerating the periodontal attachment apparatus lost to periodontal disease. Several types of membrane barriers, each one with distinct properties, have been utilized to apply this principle in periodontal wound healing. A series of case reports introduces and discusses the attributes of rubber dam as a barrier membrane for the treatment of multiple periodontal osseous defects. Comparison of preoperative and postoperative clinical measurements as well as reentry procedures are utilized to highlight successful osseous regeneration.