Aim of this study was to compare the efficacy in plaque and gingivitis reduction of an experimental toothbrush (GABA International AG, T) with a standard toothbrush (ADA reference toothbrush, C) in a splitmouth (I) and a 3 months parallel study design (II). Materials and methods: 86 male subjects took part in the study. After 48h of plaque regrowth the teeth of 2 quadrants were brushed for one minute by random with one and the remaining 2 quadrants with the other toothbrush. Immediately before and after tooth brushing (I) as well as after 3 months (II) plaque (Turesky), gingivitis (Löe & Sillness) and the number of gingival lesions = 5mm and > 5mm were evaluated by one blinded exam-iner (E.B.). After that, the individuals were appointed either to the test or the control group by ran-dom (n=43 each). All participants had to follow a standardized oral hygiene regimen for the following 3 months. Results: (I) In total, plaque was reduced from 1.95 ± 0.48 to 1.02 ± 0.41 (p Keywords: manual toothbrush