This study aimed to determine the effect of adhesive direct composite restorations, endodontic treatments, and fatigue treatments on the cuspal deflection of maxillary premolars subjected to different cyclic occlusal forces. Thirty intact maxillary second premolars were selected. Ten teeth were left untreated (group IN), 10 teeth were subjected to endodontic and restorative treatment (group FL), and the remaining 10 teeth were subjected to endodontic, restorative, and fatigue treatments (group FT). All teeth were subjected to 5 occlusal compressive loading forces (98, 147, 196, 245, and 294 N) with a universal testing device. A total of 15 experimental groups were obtained with 3 tooth conditions (IN, FL, FT) and 5 different occlusal loading values. Deflection amounts (μm) were measured with laser sensors and recorded, and obtained data were statistically analyzed with one-way analysis of variance at a significance level of .05. Mean cuspal deflection values (μm) and SDs of experimental groups ranged as follows: IN-98 (24.4 ± 19.8), IN-147 (34.8 ± 28.9), IN-196 (43.8 ± 34.7), IN-245 (54.5 ± 46.4), IN-294 (60.3 ± 50.6), FL-98 (56 ± 49.1), FL-147 (62.6 ± 49.6), FL-196 (72.4 ± 52.1), FL-245 (81.3 ± 56), FL-294 (92.2 ± 60.9), FT-98 (77.2 ± 80.9), FT-147 (83.4 ± 81.3), FT-196 (92.6 ± 83.7), FT-245 (102.7 ± 85.4), and FT-294 (124.2 ± 89.5). Mean values of three main experimental groups were as follows: IN (43.5 μm), FL (72.9 μm) and FT (96.0 μm). Significant differences were found between the three main groups and relevant subgroups (P .001). Highest cuspal deflection values (CDV) were obtained in FT groups. Lowest CDV were obtained in IN groups. FL groups showed higher deflection values than IN groups. CDV increased progressively as the teeth were restored and subjected to fatigue treatment.