Objective: This study wants to evaluate the bond strength of a self-adhesive flowable composite, in dentin surfaces prepared with Er,Cr:YSGG laser with two different settings. Methods: Fifteen healthy teeth were distributed in 3 groups. The dentin samples of the Control Group didn't have any laser treatment, the samples of the Group 2 were treated with Er,Cr:YSGG laser, with the settings 4,5W, 50 Hz, 50 ms, 70% air, 90% water, and the ones from Group 3 were treated with the same laser with a different laser frequency, 4,5W, 75 Hz, 50 ms, 70% air, 90% water. The samples were restored with VertiseTM Flow following the manufacturer instructions. The Shear Bond Strength (SBS) test and morphological analysis with Scanning Electron Microscopy (SEM) were performed. Results: The high mean of the SBS was from Group 2 (4,76 ± 3,99 MPa), followed by the Control Group (1,87 ± 1,61 MPa), and the lowest was from the Group 3 (0,81 ± 0,93 MPa). The means of SBS were not statistically different between each other. (p>0,05). Conclusion: Although the bond strength was influenced by the type of dentin surface and the laser parameters, there was not statistically differences between each group (p>0,05). The increase of the laser frequency resulted in lower SBS. Additional studies should be carried on. Schlagwörter: laser, erbium, Er, Cr:YSGG, adhesion, self-adhesive flowable composite, dentin