Purpose: To assess malocclusion and related quality of life among 13- to 19-year-old students in Mysore City. Materials and Methods: A total of 900 students aged 13-19 years selected based on multistage stratified random sampling were included in this cross-sectional study. A pre-designed format was used to record the sociodemographic details, oral hygiene practices, personal habits, malocclusion and oral health related quality of life. Malocclusion was assessed using Dental Aesthetic Index (DAI ) and quality of life using Oral Health Impact Profile-14 (OHIP-14). Further, the association between malocclusion and related quality of life was assessed. Results: Normal or slight malocclusion was seen in 79.2% (n = 713). Definite malocclusion was seen in 12.8%, severe malocclusion in 5.7% and handicapping malocclusion in 2.3% of the subjects. The overall prevalence of malocclusion was found to be 20.8%. Out of the 900 study subjects 46.6% (n = 419) reported that malocclusion had some impact on their oral health related quality of life. The results of logistic regression demonstrated that those with malocclusion experienced a significant impact on quality of life (OR = 16.07, CI = 9.17-28.17). Conclusion: Malocclusion has an impact on the oral health related quality of life among 13- to 19-year-old students in Mysore City. Keywords: adolescents, Dental Aesthetic Index, dental aesthetics, Oral Health Impact Profile