Introduction: Transmission of infectious diseases carries high risks for patients and dental professionals. Prosthetic work pieces have to be disinfected. To date, there are no specific disinfection products available for the one-shot disinfection process. Instead, universal disinfectants are used. Objective: The aim of this study was to investigate the influence of chemical disinfectants in the one-shot disinfection process on material-mechanical parameters of several denture base resins. Material and Method: 252 test specimens from four acrylic denture base resins (PalaXpress, Paladon 65, Futura Gen, FuturAcryl 2000) were produced. Test specimens were constructed prismatically according to DIN-EU-ISO 3167. Three chemical disinfecting agents were investigated (Impresept, D50 Instru-Gen, Stammopour DR). Specimens were stored in distilled water and divided into five test groups (2xcotrol, 3xtest). E-Modulus and flexural strength were detected using the three-point bending test. Results: There was no significant influence of the disinfection agents on the E-modulus detectable ((PMMA vs. distilled water from (Futura Gen) 2688.80 ± 230.78 vs. 2766.60 ± 91.22 MPa,p=0.847 to (PalaXpress) 3004.20 ± 26.40 vs. 2851.00 ± 95.23 MPa, p0.001). In determination of the flexural strength after storage in distilled water, no significant difference was measurable (PMMA vs. distilled water from (Paladon65) 27.28 ± 1.30 vs. 28.42 ± 0.84 N/mm2, p=0.216 to (PalaXpress) 30.88 ± 0.25 vs. 29.68 ± 0.79 N/mm2, p0.001). Discussion and Conclusion: After storage in distilled water and subsequent one-shot disinfection, there was no significant influence of the E-modulus and flexural strength detectable compared to the specific standard test specimen. For avoiding cross-infections and standardising of working processes, one-shot disinfections are feasible for acrylic dentures. Keywords: cross infection, chemical disinfectants, denture base resins, E-modulus, flexural strength, disinfection