Introduction: Nowadays, tooth whitening is the most conservative and more used option to remove the dental pigmentation. Objective: To evaluate the color variations (∆E) occurred in two distinct groups submitted to vitro whitening different techniques, and also to evaluate which technique achieves better clinical effectiveness. Materials and methods: Fifty four bovine teeth extracted (incisors mandibular central), were divided, randomly, into two study groups (N=24, each group). Teeth were pigmented in a coffe, black tea and cola solution. In group 1 dental bleaching was performed with Polanight 16% carbamide peroxide (SDI, Australia). In group 2 we combined Pola Office+ (SDI, Australia), hydrogen peroxide, associated with Polanight 16% carbamide peroxide. The color measurement was made with Vita Easyshade® spectrophotometer at the initial moment, after pigmentation, treatment finalization and follow-up after a week. Results: The ∆E, - after pigmentation and final - were statistically significant in both periods. It was not found statistically significant differences at the treatment finalization of treatment between group 1 and 2, however the group 2 presented a slightly higher ∆E. Conclusion: Both dental whitening treatments proved effective, however when compared, the two techniques didn't present statistically significant differences. Keywords: Dental bleaching, carbamide peroxide, hydrogen peroxide, vital tooth bleaching, home bleaching