Introduction: Periodontitis can result in irreversible loss of connective tissue and supporting alveolar bone. Despite advances in regeneration therapy, treatment of periodontal furcation defects is still a challenge. This case report describes a combined regenerative approach in the treatment of grade II furcation defects in mandibular molars. Material and Methods: In the present case study, 2 clinical cases with advanced localised periodontitis and an occlusal trauma as a cofactor were studied over 8 and 5 years, respectively. Following initial occlusal adjustment, the periodontal defects were treated successfully with guided tissue regeneration along with allogenic cancellous bone, enamel matrix proteins and endogenous growth factors. Results: The treatment was effective in the regenerative therapy of destructive periodontal disease in both patients. Significant amount of bone fill was seen in clinical and radiographic re-evaluation and clinical results were maintained in the follow-up after 8 and 5 years. Conclusion: Successful regeneration of periodontal tissues can be achieved using the combination of guided tissue regeneration (GTR), Allograft bone substitute, Emdogain and plasma rich in growth factor (PRGF). The combination therapy resulted in regeneration of tooth supporting tissue with improved clinical attachment levels and healthy gingiva. Keywords: Allograft bone substitute, Enamel matrix derivates, chronic periodontitis, guided tissue regeneration, occlusal trauma, plasma rich in growth factor