the objective of this study was to evaluate the in vitro microleakage of a new adhesive system, Syntac. Class V preparations were cut at the cementoenamel junction in 20 extracted bovine incisors and 20 extracted human incisors. One group of 10 human and 10 bovine teeth was restored with Syntac and a microfilled posterior resin composite; the other group was restored with Syntac and a hybrid resin composite, Microleakage was evaluated a 1 week with a 45 Ca radioisotope method. No statistically significant differences between human and bovine substrates were found with either resin composite. The Syntac/hybrid resin restorations leaked less than did the Syntac/microfilled resin restorations at the gingival (dentin) margin in the bovine teeth; however, this difference was not statistically significant.