A single-incision technique was introduced to minimize the size of the palatal wound and allow for primary closure of the donor site, which may result in reduced postoperative pain. It is sometimes necessary to repeatedly use the same area of a patient's palate for consecutive connective tissue grafts because of limitations caused by inadequate tissue or vascular anatomy. This case presentation describes the clinical results of 2 subepithelial grafts for root coverage from the same donor area at different times using the single-incision technique. The root coverage obtained with both the first and second connective tissue grafts from the same donor area using the single-incision technique at a 6-week interval showed predictable results, with reduction of dentin hypersensitivity without probing defect or significant complication. This approach may be an option when multiple connective tissue grafts are needed. The presented results need to be validated in further large case series or case-controlled studies. Keywords: connective tissue, multiple, palate, single incision, tissue graft