Purpose: The aim of this in vitro study was to evaluate the accuracy of template-guided implantation planned with implant-planning software (Implant Studio), comparing computer-aided design/computer-assisted manufacture (CAD/CAM)-based measurements with measurements via cone beam computed tomography (CBCT). Materials and Methods: Thirty template-guided implantations were planned and performed on acrylic-resin models. The implant positions were detected with an intraoral scanner, evaluated with CAD quality-control software, and compared with the planned positions in the test group. Preliminary deviations were measured via CBCT in the control group of the first 10 samples and compared with the first 10 samples of the test group. Results: When directly compared, measurements obtained using CBCT (control group) showed a trend toward greater deviations. In the CAD/CAM-based evaluation of the 30 samples, the mean ± SD deviation of the insertion axis from the planned implant axis was 2.011 ± 0.855 degrees. The mean deviations of the implant shoulders in the horizontal direction and at the implant apices were 0.725 ± 0.142 mm and 0.990 ± 0.244 mm, respectively. In the vertical direction, the mean deviation was 0.541 ± 0.129 mm. Conclusion: CAD/CAM-based measurements are more accurate than CBCT measurements. Therefore, this radiation-free measurement method is a viable diagnostic alternative. Implant planning with planning software and subsequent placement using surgical templates appears to be a reliable and precise therapeutic option in vitro. However, these findings will still have to be supported by in vivo studies. Keywords: dental implant, guided surgery, implant planning accuracy, intraoral scanner, navigated surgery, surgical template