Purpose: The purpose of the study was the evaluation of possible cytologic effects of taurolidine to fibroblasts and osteoblast-like cells. Materials and Methods: Human gingival fibroblasts and SaOS-2 cells were seeded on samples with sand-blasted and acid-etched surfaces. Both groups were treated with taurolidine, chlorhexidine, and pure water with three different treatment times. Three dates of measurements were set to evaluate cell viability, cytotoxicity, and apoptosis. Results: Highest cytotoxicity was measured in both cell lines in the groups treated with chlorhexidine, while cell viability was lower than in the corresponding taurolidine and pure water groups; on days 3 and 6 these differences were significant. Taurolidine showed similar results to the pure water groups. Conclusion: The results of this study indicate that taurolidine is biocompatible and gentle to the tested human cells for the application time of a mouthrinse. Keywords: peri-implantitis, cell culture, antiseptic treatment