Purpose: To investigate the influence of dishevelled-2 (Dvl2) protein and the phosphorylation and ubiquitination of Dvl2 on dental implant osseointegration in hyperlipidemic rats. Materials and Methods: Thirty-six Wistar rats were divided equally into two groups: an experimental group fed with a high-fat diet for 8 weeks and a control group fed with a normal diet for 8 weeks. Serum lipids were detected, and titanium implants were placed into bilateral proximal metaphysis of rats' femurs. At 1, 3, and 5 days after implantation, rats were sacrificed, and 1 mm of bone around each implant was obtained. Real-time polymerase chain reaction of runt-related transcription factor 2 (Runx2), Cathepsin K, and Dvl2 was performed. Western blot of Dvl2, phosphorylation, and ubiquitination of Dvl2 was also performed. Results: In the experimental group, the expressions of Runx2, Dvl2, and phosphorylation of Dvl2 were lower (P .05), while that of Cathepsin K and ubiquitination of Dvl2 were higher than those in the control group (P .05). Conclusion: Hyperlipidemia suppressed the dental implant osseointegration. This inhibition may be modulated by downregulated Dvl2, phosphorylation of Dvl2, and upregulated ubiquitination of Dvl2. Keywords: dishevelled-2, hyperlipidemia, implant osseointegration, rats, Wnt signaling pathway