Objectives: The study was performed to evaluate the influence of dentin perfusion on dentin bond strength of three different dentin adhesives (Excite, Clearfil New Bond and an experimental self-conditioning adhesive) in vitro. Methods: Sixty freshly extracted third molars, stored in saline for a maximum of seven days after extraction were included. All teeth were specially prepared allowing the simulation of dentin perfusion. Dentin specimens with a total thickness of 3.5 mm were obtained under standardized conditions. The specimens were randomly assigned to six experimental groups of ten samples each: Group EM: Excite (moist dentin), group EP; Excite (perfused dentin); group CM: Clearfil (moist dentin), group CP: Clearfil (perfused dentin); group XM: experimental adhesive (moist dentin), group XP: experimental adhesive (perfused dentin). Tensile bond strength of the above mentioned bonding agents was measured 15 minutes after application and light curing of the composite material (Tetric Ceram, colour A2) using an universal testing machine. Results: For the six test series following tensile bond strengths were evaluated (mean value and standard deviation in MPa): group EM 4.09 (± 1.56), group EP 3.92 (± 1.34), group CM 4.68 (± 2.41), group CP 3.18 (± 0.96), group XM 3.14 (± 1.34) and group XP 2.38 (± 0.69). Statistical analysis showed a significant influence of the used dentin bonding agent on tensile bond strength (p Conclusions: The results of this investiation suggest that newer dentin adhesives are not affected by dentin perfusion to the extends described in previous studies. Keywords: dentin, adhesion, bond strength, perfused dentin, dentin bonding agent