Objectives: The aim of this study was to compare microtensile bond strength of a self-etching resin cement (Bifix SE, Voco, Germany) with an established resin cement system (Bifix, Futurabond DC, Voco, Germnay) on different root canal dentin localizations (coronal, cervical and apical) in vitro. Methods: In this study 90 single rooted extracted teeth, stored in saline for a maximum of thirty days after extraction were used. All teeth were specially prepared allowing the evaluation of microtensile bond strength on root canal dentin. The specimens were randomly assigned to six experimental groups of fifteen samples each: Group 1: Bifix/ coronal dentin; group 2: Bifix/ cervical dentin; group 3: Bifix/ apical dentin; group 4: Bifix SE/ coronal dentin; group 5: Bifix SE/ cervical dentin; group 6: Bifix SE/ apical dentin. Microtensile bond strength was measured 15 minutes after application using an universal testing machine (Z005, Zwick). All materials were applied as recommended by the manufacturer and light-cured for 60 seconds. Results: The material combination Bifix with Futurabond DC showed in coronal region 34.7 MPa, in cervial region 30.1 MPa and in apical region 30.9 MPa. In the case of Bifix SE in coronal dentin 48.8 MPa, in cervical dentin 37.0 MPa and in apical region 30.4 MPa were evaluated. Regarding both resin cements, Bifix SE showed singnificantly higher bond strengths on coronal and cervical dentin (p Keywords: resin cements, microtensile bond strength, root canal dentin, endodontic irrigation solutions