Objectives: The aim of this study was to evaluate microtensile bond strength of a self-etching resin cement (Bifix SE, Voco, Germany) after irrigation with different endodontic irrigants (0.2% chlorhexidine, 1% sodium hypochloride, 19% EDTA) on root dentin in vitro. Methods: 75 single rooted extracted teeth, stored in saline for a maximum of fourteen days after extraction were included. All teeth were specially prepared allowing the evaluation on root canal dentin. The specimens were randomly assigned to five experimental groups of fifteen samples each: Group 1: Control, Bifix SE/ no irrigation solution; group 2: Bifix SE/ chlorhexidine (CHX); group 3: Bifix SE/ sodium hypochloride (NaOCl); group 4: Bifix SE/ chlorhexidine and sodium hypochloride; group 5: Bifix SE/ EDTA. The different solutions were applied five times for 2 minutes each. Microtensile bond strength was measured 15 minutes after application using an universal testing machine (Z005, Zwick). All materials were applied as recommended by the manufacturer and light-cured for 60 seconds. Results: For the five test series following microtensile bond strengths were evaluated (mean value and standard deviation in MPa): Group 1: 37.0 (±2.8); group 2: 27.5 (±3.6); group 3: 27.6 (± 2.8); group 4: 27.5 (± 3.0); group 5: 42.3 (± 3.2). Bifix SE showed the highest bond strengths after the use of EDTA as iirgation solution (p Keywords: resin cements, tensile bond strength, dentin bonding, dentin, endodontic irrigation solutions