Purpose: The aim of this study was to evaluate in vitro the influence of fiber reinforcement and luting cement on the static failure load (SFL) and dynamic failure load (DFL) of simulated two-unit cantilever resin-bonded fixed dental prostheses (RBFDPs). Materials and Methods: Forty-six particulate filler composite (PFC) beams and 76 fiber-reinforced composite (FRC) beams were prefabricated and subsequently luted (RelyX ARC or Panavia F2.0) onto flat ground bovine enamel. The SFL of the different specimen types was determined with a peel test and the DFL was determined with a rotating cantilever beam fatigue testing device. Results: The PFC specimens showed a significantly lower SFL than the FRC specimens. The luting cement showed a significant effect on the SFL of the PFC specimens, but not with FRC. The DFL of PFC specimens was significantly lower than for FRC specimens. The luting cement showed a significant effect on the DFL of the PFC specimens, but not so with FRC. With both the SFL and the DFL tests all PFC beams fractured, leaving the bonded part on the tooth surface, but FRC beams partially debonded from the tooth surface, leaving fibers connected to the enamel surface to a varying extent. Coincidentally, the uncured fibers turned out to be prone to aging, an effect which has been investigated. Conclusion: Within the limitations of this study, it can be concluded that PFC without fiber reinforcement is not suitable for the fabrication of two-unit cantilever RBFDPs, despite the significant effect of the luting cement, but FRC is suitable. Keywords: fiber-reinforced composite, particulate filler composite, cantilever, failure load, fatigue