Purpose: To evaluate the influence of pH cycling on microtensile bond strength (µTBS) and fracture pattern of MDPB- and fluoride-containing self-etching adhesive systems to dentin, and on the cross-sectional Knoop microhardness (CSMH) of enamel and dentin adjacent to restorations. Materials and Methods: The two-step self-etching adhesive Clearfil SE Bond (SE; Kuraray), the two-step MDPBand fluoride-containing adhesive Clearfil Protect Bond (PB; Kuraray), and the one-step fluoride-containing adhesive One-Up Bond F Plus (OU; Tokuyama) were used to bond resin composite to midcoronal dentin surfaces (for µTBS testing) or to Class V cavities (for CSMH testing). µTBS and CSMH tests were performed after a 15-day period of pH cycling or storage in artificial saliva. Results: µTBS to dentin was not affected by pH cycling or storage in artificial saliva; however, µTBS values found for PB were higher than those observed for OU. No difference existed among the µTBS values shown by PB, OU, and SE. The fracture pattern was affected by both pH cycling and adhesive system. In enamel, there was no difference in CSMH values provided by the different adhesive systems and storage media, regardless of the distance and depth from restoration. In dentin, PB and SE showed the highest CSMH values, which differed from those obtained for OU. Significantly higher CSMH values were found 100 µm from the restoration margin for all adhesive systems tested. Conclusion: The bond strength and microhardness in the vicinity of restorations were adhesive dependent, with MDPB and fluoride exerting no effect on the performance of the adhesive systems. Keywords: adhesion, bond strength, self-etching adhesive systems, pH cycling