Purpose: To investigate the effects of pretreatment with 2% chlorhexidine in a gel base (CHX) and 100% ethanol (EtOH) on the bond strength between fiber posts relined with resin composite and root dentin under cyclic loading. Materials and Methods: Forty bovine incisor roots were divided into four groups after phosphoric acid etching: group 1 (control), irrigation with physiological saline solution; group 2, 5 min pretreatment with CHX; group 3, 1 min pretreatment with EtOH; group 4, 5 min pretreatment with CHX followed by 1 min with EtOH. Fiber posts relined with resin composite were cemented with RelyX ARC and the etch-and-rinse adhesive system Scotchbond Multi-Purpose. Each group was randomly divided into two subgroups: 24 h of storage (immediate groups) and cyclic loading (loading groups) with 250,000 cycles in a controlled chewing simulator. All roots were sectioned transversely and push-out tests were performed. Failure modes were observed and the bond strength means were analyzed using ANOVA and Tukey's test (a = 0.05). Results: The mean values for the bond strength test (MPa) in immediate groups were: group 1, 5.44 ± 1.48; group 2, 5.57 ± 1.41; group 3, 5.49 ± 1.48; group 4, 5.57 ± 1.42. Immediate groups showed similar bond strength values (p > 0.05). In the cyclic loading groups, the bond strength values were: group 1, 2.80 ± 0.79; group 2, 4.02 (1.30); group 3, 4.50 ± 1.67; group 4, 4.97 ± 2.00. After cyclic loading, a significant decrease in the control group was observed (p 0.05), while CHX pretreatment resulted in intermediate values (p 0.05) and EtOH alone or associated with CHX preserved the bond strength values (p > 0.05). Conclusion: Chlorhexidine and/or ethanol pretreatment preserved the bond strength of the fiber post after cyclic loading. Keywords: bond strength, chlorhexidine, cyclic loading, ethanol, fiber post, root canal