Purpose: The aim of the present study was to measure the bond strength of adhesively luted glass-fiber bundles inside the root canal with respect to the application procedure in comparison to conventional solid glass-fiber posts. Materials and Methods: 104 human anterior teeth were endodontically treated, root filled and divided into 8 groups (n = 13). After post space preparation, fiber bundles consisting of 6 and 12 glass fibers, respectively, were luted adhesively with a multi-mode adhesive (Futurabond U; Voco, Cuxhaven, Germany) and a dual-curing composite (Rebilda DC, Voco) with the following application modes into the root canal: (1) direct application with tweezers, (2) distribution of the fibers using a spreader, (3) application of ultrasound after insertion of fibers. Two different solid posts (Rebilda DC, Voco; and DentinPost, Komet, Lemgo, Germany) were used as controls. Roots were sectioned into 6 slices per root (thickness 1 mm). Bond strengths were measured using thin-slice push-out tests for 3 slices 24 h after post insertion and for 3 slices per sample following thermocycling (TC) for 6000 cycles and storage in 0.9% NaCl for 6 months. Homogeneity of the slices was analyzed using a stereomicroscope and, for representative samples, micro-computed tomography (µCT). Results: Mean push-out bond strengths (MPa) were significantly affected by post system (p 0.0005) and location inside the root canal (p = 0.004) but not by application mode (p = 0.544) or TC (p = 0.098; repeated measurement ANOVA). Fiber bundles consisting of 6 (13.2 ± 4.7) and 12 fibers (14.5 ± 4.3) revealed bond strength comparable to that of Rebilda Post (13.67 ± 3.2) but significantly higher than that of Dentin Posts (8.7 ± 3.02). Inhomogeneities were detected among 35.5% to 43.1% of the fiber-bundle samples, irrespective of number of fibers and application mode, and among 24.4% to 27.3% of the solid posts (p = 0.010; chi-squared test). µCT revealed voids inside the composite bulk between the fibers as well as between composite and dentin of adhesively luted fiber bundles. Conclusion: Adhesively luted fiber bundles achieved bond strengths comparable to those of solid fiber posts for one investigated post type, and even higher values compared to another post type. Inhomogeneities were frequently detected irrespective of application mode. Keywords: root canal dentin, fiber post, fiber bundles, aging, adhesive luting