Purpose: To use a novel approach to measure the amount of vertical marginal gap in computer numeric controlled (CNC)-milled titanium frameworks and conventional cast frameworks. Materials and Methods: Ten cast frameworks were fabricated on the mandibular master casts of 10 patients. Then, 10 CNC-milled titanium frameworks were fabricated by laser scanning the cast frameworks. The vertical marginal gap was measured and analyzed using the Contura-G2 coordinate measuring machine and special computer software. Results: The CNC-milled titanium frameworks showed an overall reduced mean vertical gap compared with the cast frameworks in all five analogs. This difference was highly statistically significant in the distal analogs. The largest mean gap in the cast framework was recorded in the most distal analogs, and the least amount was in the middle analog. Conclusions: Neither of the two types of frameworks provided a completely gap-free superstructure. The CNCmilled titanium frameworks showed a significantly smaller vertical marginal gap than the cast frameworks.