Purpose: To investigate whether hemostatic agents have any detrimental effects on polyether impressions and plaster casts after a clinically viable cleaning protocol. Materials and Methods: A total of 60 human molar teeth were manually standardized, prepared, and subdivided into five groups (G): G0 (control/ no contamination); G1 (ViscoStat Clear); G2 (ViscoStat); G3 (Astringedent); and G4 (Astringedent X). Each tooth was contaminated with a hemostatic agent for 3 minutes and cleaned with a 30-second air/water spray. Before and after contamination, an impression was taken and a plaster cast was poured. The impressions and plaster casts of all teeth were scanned using a light scanner. Data were matched using a three-dimensional analysis software that displayed color-coded maps. Means of the maximum positive/negative values and of the mean deviations in each group were calculated and compared. All color-coded maps were interpreted descriptively using the program's color scale, and all impressions and plaster casts were microscopically evaluated. Results: Values of mean deviations throughout all matched scans showed maximum values ranging from -30.1 to +17.5 μm. None of the groups showed significantly different deviation values or patterns when compared to each other. Impressions showed a thin, discontinuous coating for G2, G3, and G4; no changes were found for plaster casts. Conclusion: Presented deviations showed the same values and distribution patterns within all groups. A cleaning protocol of 30 seconds of air/water spray is sufficient to remove hemostatic agents, preventing effects to polyether impressions and plaster casts. However, ferric sulfate-containing hemostatic agents left discontinuous coatings on impression surfaces.