The purpose of this study was to evaluate the results of treatment of buccal mandibular Class II furcation defects with a combination of autologous bone grafts and enamel matrix derivative over a 24-month period. Eleven individuals with chronic periodontitis were selected and contributed one furcation defect each. Statistically significant improvements in mean vertical probing attachment level and horizontal probing attachment level were obtained at the 2-year evaluation. Complete clinical closure was achieved in four sites, which exhibited favorable defect morphology for this regenerative procedure. All other residual defects were reduced to Class I.