Objective: The purpose of this study was to compare the solubility, pH, calcium ion release and setting time of Biodentine, Bioactive glass, nano Biodentine and nano Bioactive glass. Materials and Methods: For solubility, pH changes and calcium ion release, the tested specimens were immersed in distilled water. The evaluation was performed after 1 h, 24 h and 7 days. The pH values were measured using a digital pH meter. The measurement of calcium ion release was completed using atomic absorption spectrophotometry. The setting time was measured using a Vicat apparatus. Data were analysed by the One Way ANOVA test followed by the Tukey post-hoc test at P ≤ 0.05. Results: Nano Biodentine showed significant highest mean pH values up to the seventh day, highest calcium ion release at all experimental periods and the shortest setting time (P ≤ 0.001). On the other hand it showed the greatest solubility, while Bioactive glass and nano Bioactive glass showed negative solubility. Conclusions: Nano Biodentine showed the highest alkalinizing action, offered a superior affinity to release calcium ions and a distinct short setting time, but with high solubility. Keywords: Bioactive glass, Biodentine, nano Bioactive glass, nano Biodentine, physicochemical properties