Sphaerobacter thermophilus, a Gram-positive, non-motile & non-spore forming bacteria is reportedly present in teeth and produces glycosyltransferase, which is responsible for endodontic infection. The enzyme is found in the human root canal and is involved in peptidoglycan biosynthesis that is significant for bacterial adhesion and biofilm production. In the present study, relevant databases & webservers were accessed for in silico proteomic characterization, virulence and protein disorder prediction to support the metabolic pathway design. Glycosyltransferase-1 family members were phylogenetically investigated, which are exclusively endodontic in origin. Subsequently, the enzyme was subjected to a subcellular localization-based pathogenicity study using computational algorithms & an already established pathway database. Afterwards a putative metabolic pathway was engineered & presented that may further lead towards exploration of glycosyltransferases with wider range of chemical configurations and competent drug designing.