A simple method to measure candidal activity using pH change of Stomastat was developed and used to evaluate the efficacy of 11 commercial denture cleansers on Candida albicians biofilm. The initial number of yeasts inoculated correlated with the pH value of Stomastat after both 24- and 30- hour incubation periods (r=.992; 4 = .988, respectively; P.01), which supported the method's validity. The ability of the cleansing agents to decrease fungal biofilm activity varied depending upon the components of the agents. In general, peroxide denture cleansers, a disinfectant, and one enzyme cleanser were more efficacious than the other types tested in this study.