This study aimed to evaluate the effect of gender, antagonistic teeth, and type of denture on clinical wear of denture teeth. Wear measurements were made on gypsum replicas of 204 posterior denture teeth after 6 months using an optical 3-dimensional profilometer. Data were analyzed using univariate and mixed regression procedures. The mean wear of all denture teeth was -20.5 µm (± 14.6). Males demonstrated significantly higher wear than females. Interactions of the variables "gender" and "type of denture" and "antagonist material" and "type of denture" also showed significant influences on wear. The results indicate that some clinical parameters may affect wear of denture teeth.