Purpose: This in vitro study evaluated the effect of biofilms on abutment torque loss and wear of implant internal connection surfaces. Materials and Methods: Morse taper abutments were torqued to corresponding implants and then the implant-abutment assemblies were immersed in a biofilm medium for 72 hours. After detorque evaluation, the abutments were removed and the inner implant surfaces were observed via scanning electron microscopy and profilometry. Results: The removal torque values and the implant damaged areas decreased after contact with biofilms. Conclusion: The lubricating effect of biofilms decreased the friction between contacting surfaces, negatively affecting the mechanical integrity of the implant-abutment connection.